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C O N T E N T S
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 Supported, in part, by the National Science Foundation - Division of Undergraduate Education
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Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
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 C-Fern Web Manual - Culture of Gametophytes

  germinating C-Fern spores
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Spore Germination and Gametophyte Development

Under standard temperature and light conditions, spore germination is evident three to four days following spore soaking (DFS) and complete by six to eight DFS. Male and hermaphroditic gametophytes are sexually mature at 10 to 12 DFS. This is the best time to observe fertilization or to perform cross or self fertilization for sporophytes. In older gametophytes effective spermatozoid production decreases.