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C-Fern Logo  C-Fern
C O N T E N T S
C-Fern Home
Web Manual
  Introduction
  C-Fern Nutrient Medium
  Culture Conditions
  Gametophyte Culture
  Gametophyte Observations
  Sporophyte Culture
  Spore Mutagenesis
  Bibliography

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cfern@utk.edu
 Supported, in part, by the National Science Foundation - Division of Undergraduate Education
Development of C-Fern is supported
by the National Science Foundation (NSF-DUE)
Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
All rights reserved.
 C-Fern Web Manual - Culture of Gametophytes

  Hermaphroditic and male C-Fern gametophytes
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Temperature

The temperature optimum for spore germination and gametophytic development is about 28 C (82 F) as measured inside the Culture Dome or C-Fern Growth Pod. This is somewhat higher than for many other plants. Similar growth and development of gametophytes can be obtained at 26-30 C (79-86F). However, lower temperatures will substantially alter developmental timing; for example, at 20 C (68 F) development times will be increased by about two-fold. It is a good idea to monitor and record the temperature inside Growth Pods or Culture Domes daily. Some control of the temperature inside the Growth Pods or Culture Domes can be achieved by adjusting the distance between the light source and C-Fern cultures. Once a suitable temperature is achieved, the height of the lights should remain constant during all phases of culture.