![]() ![]() |
|
| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
|
Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
| Web Journal |
| Educational Resources |
|
National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
![]() |
| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
|
Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
| C-Fern
Sport Report
Ghost-like pal1 gametophytes appear to have decreasing amounts of chlorophyll as they enlarge. Nonetheless, they grow to sexual maturity within two weeks and can self or cross-fertilize. At 1-2 weeks, pal1 gametophytes are slightly smaller than wild type and their pale phenotype makes them clearly recognizable in a segregating population (use of transmitted light is best). This is a recessive mutation in sporophytes and leaves of pal1/pal1 homozygotes show a yellow –green phenotype when viewed with reflected light (from the side or top). These attractive sporophytes are quite viable, but slower growing than wild type. This EMS-induced mutation is available only in an F1 hybrid stock. The F1 spores can be used very effectively in the Genetics in Action Kit (15-6708), as a substitute for polka dot (cp). |