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C O N T E N T S
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 Supported, in part, by the National Science Foundation - Division of Undergraduate Education
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Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
All rights reserved.
 C-Fern Web Manual - Observation and Analysis of Gametophytes

 
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Acetocarmine

45% Glacial acetic acid 100 mL
Carmine 0.5 g

Boil acetic acid and carmine gently for 5 minutes in a beaker covered with a watch glass. Do this in a fume hood! Shake while the mixture cools. Filter cooled solution through #2 Whatman paper. Filtration takes a long time and several changes of paper are usually necessary. Store stain in brown glass at room temperature.